WebJun 19, 2024 · A pc-DNA3.1 plasmid was designed to induce overexpression of GLS1, and the cells were divided into the pc-DNA3.1-GLS1-transfected cell group; the NC group, which was transfected with an empty pc-DNA3.1 vector; and the mock group, which consisted of untransfected cells. The siRNA sequences were as follows: siRNA1, ... WebTwo independent experiments were carried out using the protocol described by Paiva for juveniles [], however, adults were used in this study.For Experiment 1, we used 85 wild-type, shortfin zebrafish (Danio rerio) of heterogeneous backgrounds and both sexes, which were obtained from Ecofish (Minas Gerais, Brazil).Animals were 4–5 months old and …
pcDNA 3.1/myc-His(-) A, B, and C - Thermo Fisher Scientific
WebMar 26, 2015 · DNA 3 Summary. From DNA 3 Condos: With the tremendous success of the DNA1 and DNA2 buildings, Canderel Stoneridge is set to push the envelope once again … WebA. O. Maslova and I. M. Hsing, Nanoscale Adv., 2024, 1, 430 DOI: 10.1039/C8NA00148K This article is licensed under a Creative Commons Attribution 3.0 Unported Licence . … shelter charity shop bolton
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WebOct 29, 2024 · HeLa cells transiently transfected with pc-DNA3.1-DDB2 Wt or pc-DNA3.1-DDB2 PCNA-constructs and UV-C local irradiated were analysed 30 min and 1 h after … http://dnairthree.com/ WebAll pcDNA™ vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in ... sports direct in plymouth