WebAn enzyme with a high Km has a low affinity for its substrate, and requires a greater concentration of substrate to achieve Vmax." The importance of determining Km and … WebEnzymes with high Km's can be much faster in reaction velocity. Think of it as also rate of release of product when affinity is high. For e.g. if products also bind strongly their …

Affinity and activity of enzyme substrate interaction?

WebThe specific activities of purified enzyme preparations were 9.1, 1.0, 0.4 and 0.5 mumols/min/mg protein of AMP deaminase, "high Km" 5'-nucleotidase and adenosine … Web13 de fev. de 1981 · It has a high affinity for mannose and fructose and low affinity for glucose. Km values are 6, 0.07 and 1.2 mM and relative maximum rates 100, 520 and 1070 for glucose, mannose and fructose, respectively. Hexokinase I is not inhibited by physiological concentrations of ATP nor by glucose-6-P , mannose-6-P or fructose-6-P … can stratas borrow money in b.c

Catalytic Efficiency of Enzymes - Chemistry LibreTexts

WebGLUT2 has high capacity for glucose but low affinity (high KM, ca. 15–20 mM) and thus functions as part of the "glucose sensor" in the pancreatic β-cells of rodents, though in human β-cells the role of GLUT2 seems to be a minor one. [7] It is a very efficient carrier for glucose. [8] [9] GLUT2 also carries glucosamine. [10] There are over 10 different types of glucose transporters; however, the most significant for study are GLUT1-4. GLUT1 and GLUT3 are located in the plasma membrane of cells throughout the body, as they are responsible for maintaining a basal rate of glucose uptake. Basal blood glucose level is approximately 5mM (5 millimolar). The Km value (an indicator of the affinity of the transporter pro… WebSo when there is high km there is low affinity for its substrate and when there is low km there is high affinity for its substrate. Is this because 1) at high km (because km is … fla return online filing