WebNov 11, 2024 · Considering that M. oryzae ruptured the cuticle of the host through the mechanical force imposed by high appressorium turgor, we evaluated the appressorium turgor pressure using the incipient cytorrhysis test as described previously [Citation 36]. WebNov 16, 2024 · An incipient cytorrhysis assay was carried out by allowing appressoria to form in water on borosilicate cover slips for 24 hr, after which the water was replaced with a range of aqueous glycerol ranging from 0.25 to 2.5M and, after 30 min, the frequency of cytorrhysis determined (de Jong et al. 1997).
Disruption of MoCMK1, encoding a putative calcium
WebTherefore, cytorrhysis assays were subsequently performed using polyethylene glycerol 8000 (PEG8000), which has a larger molecular size than glycerol, as the external inductive … WebApr 11, 2014 · We applied incipient cytorrhysis to investigate the appressorial turgor pressure of P. pachyrhizi. Mature appressoria were … church end estate london
Fig. 1. Incipient cytorrhysis assays with appressoria formed by …
WebDec 1, 2010 · Analysis of appressorial turgor pressure by incipient cytorrhysis. (A) Collapse of appressoria caused by hyperosmolarity of the medium is indicated by ridges of the appressorial cell wall (white arrowheads). The micrographs show appressoria in 2 m glycerol. In a control without glycerol (water control), all appressoria were turgescent. Bar, … WebJul 20, 2010 · Initially, the hygromycin-resistant transformants were identified by PCR with CMK-check-p1 and CMK-check-p2. The candidate mutants were purified through single conidia isolation and DIG high-prime DNA labeling, and detection starter kit I (Roche, Germany) was used to confirm the single integration event of the mutants. WebWe applied incipient cytorrhysis to investigate the appressorial turgor pressure of P.pachyrhizi. Mature appressoria were placed in sorbitol solu- tions of increasing … deutschlandfunk podcast the cure